Isopycnic centrifugation of sheared chromatin in metrizamide gradients
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چکیده
منابع مشابه
Complex of DNA with chromatin proteins investigated by isopycnic centrifugation in metrizamide.
Complexes of mouse main band DNA with a fraction of non-histone proteins (NHP), having a high affinity for DNA, in the absence or presence of histones have been investigated by gradient centrifugation in metrizamide. Two types of complexes were formed at an input ratio of NHP to DNA between 1 and 2.5. In metrizamide gradients a majority of DNA was found in the light complex (at the density of 1...
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Solutions of Renografin (30-60%) can be centrifuged to form density gradients in the range from 1.0 g/cm(3) to 1.4 g/cm(3) or, alternatively, preformed gradients can be made which under appropriate conditions of centrifugation have an indefinite stability. Such solutions have a low viscosity and a relatively low ionic strength. The density of DNA in such solutions is surprisingly low ( approxim...
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A preparation, similar to the light mitochondrial fraction of rat liver (L fraction of de Duve et al, (1955, Biochem. J. 60: 604-617), was subfractionated by isopycnic centrifugation in a metrizamide gradient and the distribution of several marker enzymes was established. The granules were layered at the top or bottom of the gradient. In both cases, as ascertained by the enzyme distributions, t...
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Fritsch, A. (1973) Anal. Biochem. 35,57-71 Griffith, 0. M. (1978) Anal. Biochem. 9 0 , 4 3 5 4 4 3 Hames, B. D. & Rickwood, D. (1978) in Centr&gation-A Practical Approach (Rickwood, D., ed.), pp. 69-80, Information Retrieval. London Ridge, D. (1978) in Centrifugal Separations in Molecular and Cell Biology (Birnie, G. D. & Rickwood, D., eds.), pp. 33-61. Butterworths, London Young, B. D. (1978) ...
متن کاملIsolation of newly replicated chromatin by using shallow metrizamide gradients.
The properties of chromatin containing newly synthesized DNA and protein were investigated. A fraction of chromatin enriched in newly replicated DNA was isolated by means of its increased density in metrizamide relative to bulk chromatin. The DNA of this fraction appeared to be packaged into nucleosomes but at a reduced nucleosomal spacing. Although pulse-labeled DNA was present in this dense f...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1973
ISSN: 0014-5793
DOI: 10.1016/0014-5793(73)80197-8